Emergence of Plasmid-Mediated CMY-2 beta -Lactamase Produced by Clinical Isolates of Escherichia coli in Korea / 대한진단검사의학회지
The Korean Journal of Laboratory Medicine
;
: 98-103, 2005.
Article
in Korean
| WPRIM
| ID: wpr-156953
ABSTRACT
BACKGROUND:
Of the plasmid-mediated AmpC beta-lactamases (ABLs), CMY-2 is the most prevalent and is distributed in many countries. However, little is known about the emergence and characteristics of CMY-2 among Escherichia coli isolates in Korea. The aims of this study were to detect the emergence of the CMY-2 beta-lactamase in clinical isolates of E. coli from various regions in Korea.METHODS:
Eighteen cefoxitin non-susceptible isolates of 1, 130 consecutive, nonrepeat isolates of E. coli at five university hospitals were tested for antimicrobial susceptibility by the broth microdilution method. The cefoxitin non-susceptible isolates were further investigated by AmpC disk tests, double disk synergy (DDS) tests, isoelectric focusing, CMY-2-specific PCR, DNA sequencing, and plasmid analysis.RESULTS:
Seven (0.6%) isolates of plasmid-mediated ABL-producing E. coli were found at three of the five hospitals; all seven isolates produced CMY-2 beta-lactamase and one of the isolates was also tested positive by the DDS test. All isolates demonstrated different plasmid patterns by plasmid analysis.CONCLUSIONS:
Our data indicate that CMY-2-producing E. coli has emerged and is prevalent in the medical institution in Korea. Therefore, constant surveillance is needed to prevent its further spread.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Beta-Lactamases
/
Cefoxitin
/
Polymerase Chain Reaction
/
Sequence Analysis, DNA
/
Escherichia coli
/
Hospitals, University
/
Isoelectric Focusing
/
Korea
Country/Region as subject:
Asia
Language:
Korean
Journal:
The Korean Journal of Laboratory Medicine
Year:
2005
Type:
Article
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