Cellular responses on anodized titanium discs coated with 1 alpha,25-dihydroxyvitamin D3 incorporated Poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles / 대한치과보철학회지
The Journal of Korean Academy of Prosthodontics
;
: 620-627, 2008.
Article
in English
| WPRIM
| ID: wpr-157289
ABSTRACT
STATEMENT OF PROBLEM:
A biochemical approach for surface modification has offered an alternative for physicochemical and morphological methods to obtain desirable bone-implant interfaces.PURPOSE:
The purpose of the present study was to investigate cell responses to poly (D,L-lactide-co-glycolide) (PLGA)/1alpha, 25-(OH)2D3 coating with reference to cellular proliferation and differentiation in vitro. MATERIAL ANDMETHODS:
96 titanium discs were fabricated and divided into four groups. Group 1 was anodized under 300 V as control. Group 2, 3 and 4 were anodized then coated with 3 ml PLGA/1alpha, 25-(OH)2D3 solutions. Amount of the solutions were 2 ul, 20 ul and 200ul respectively. The osteoblast-like Human Osteogenic Sarcoma (HOS) cells were seeded and cultured for 1, 3 and 7 days. MTSbased cell proliferation assay and ALPase activity test were carried out.RESULTS:
PLGA nanoparticles were observed as fine, smooth and round and HOS cells attached to the anodized surfaces through strand-like and sheet-like filopodia. After 3 days of culture, the dendritic filopodia were exaggerated and sheet-like cytoplasmic projections covered the coated titanium surfaces. After 3 days of culture, all of the groups showed increased cellular proliferation and the lowest proliferation rate was measured on group 2. Higher amount of incorporated 1 alpha, 25-(OH)2D3 (Group 3 and 4) improved cellular proliferation but the differences were not significant statistically (P > .05). But they increased the rate of ALP activities than the control group at day 3 (P < .05).CONCLUSION:
Biodegradable PLGA nanoparticles incorporated with vitamin D metabolite positively affected proliferation and differentiation of cells on the anodized titanium surface.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polyglycolic Acid
/
Pseudopodia
/
Seeds
/
Titanium
/
Vitamin D
/
Calcitriol
/
Osteosarcoma
/
Lactic Acid
/
Cytoplasm
/
Cell Proliferation
Limits:
Humans
Language:
English
Journal:
The Journal of Korean Academy of Prosthodontics
Year:
2008
Type:
Article
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