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Characterization and Prognostic Value of Mutations in Exons 5 and 6 of the p53 Gene in Patients with Colorectal Cancers in Central Iran
Gut and Liver ; : 295-302, 2013.
Article in English | WPRIM | ID: wpr-158237
ABSTRACT
BACKGROUND/

AIMS:

We aimed to investigate the relation-ships among various mutations of the p53 gene and their protein products, histological characteristics, and disease prognosis of primary colorectal cancer in Isfahan, central Iran.

METHODS:

Sixty-one patients with colorectal adenocarcinoma were enrolled in the study. Mutations of the p53 gene were detected by single-stranded conformation polymorphism and DNA sequencing. The protein stability was evaluated by immunohistochemistry. Patients were followed up to 48 months.

RESULTS:

Twenty-one point mutations in exons 5 and 6 were detected in the tumor specimens of 14 patients (23%). Of those, 81% and 9.5% were missense and nonsense mutations, respectively. There were also two novel mutations in the intronic region between exons 5 and 6. In 11 mutated specimens, protein stability and protein accumulation were identified. There was a relationship between the type of mutation and protein accumulation in exons 5 and 6 of the p53 gene. The presence of the mutation was associated with an advanced stage of cancer (trend, p<0.009). Patients with mutated p53 genes had significantly lower survival rates than those with wild type p53 genes (p<0.01).

CONCLUSIONS:

Mutations in exons 5 and 6 of the p53 gene are common genetic alterations in colorectal adenocarcinoma in central Iran and are associated with a poor prognosis of the disease.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Prognosis / Immunohistochemistry / Introns / Colorectal Neoplasms / Adenocarcinoma / Exons / Survival Rate / Genes, p53 / Sequence Analysis, DNA / Point Mutation Type of study: Prognostic study Limits: Humans Country/Region as subject: Asia Language: English Journal: Gut and Liver Year: 2013 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Prognosis / Immunohistochemistry / Introns / Colorectal Neoplasms / Adenocarcinoma / Exons / Survival Rate / Genes, p53 / Sequence Analysis, DNA / Point Mutation Type of study: Prognostic study Limits: Humans Country/Region as subject: Asia Language: English Journal: Gut and Liver Year: 2013 Type: Article