Production of Monoclonal Antibody to Chlamydia Pneumoniae / 감염
Korean Journal of Infectious Diseases
;
: 139-146, 1997.
Article
in Korean
| WPRIM
| ID: wpr-15868
ABSTRACT
BACKGROUND:
Chlamydia pneumoniae is a recently recognized species consisting of the strains commonly referred to as TWAR. These strains are associated with acute respiratory infections in humans, especially atypical pneumonia. So we tried to make a monoclonal antibody to Chlamydia pneumoniae.METHODS:
C. pneumoniae were adapted to grow in HeLa-229 cells. The organisms were harvested and purified in a linear gradient of renograffin. BALB/c mice (female, 10weeks) were intravenously immunized with purified C. pneumoniae(TW-183).The spleen cells and SP 2/0 myeloma cells were fused with 40% polyethylene glycol (Mol.Wt.1,450). Antibodies against C. pneumoniae were screened by an enzyme- linked immunosorbent assay (ELISA). The proteins of purified chlamydial elementary bodies were separated by discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and Western blots were performed with these monoclonal antibodies.RESULTS:
Two monoclonal antibodies (HYMD10, HYMG12) reacted specifically with C. pneumoniae, as measured by an ELISA and indirect immunofluorecent stain. One of the monoclonal antibody (HYMD10) reacted with 75- and 39-KDa proteins in Western blot. The other monoclonal antibody (HYMG12) reacted with 98- and 39-KDa proteins of C. pneumoniae.CONCLUSIONS:
These species-specific monoclonal antibodies (HYMD10, HYMG12) to C. pneumoniae could be used for diagnosis of C. pneumoniae infections.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pneumonia
/
Polyethylene Glycols
/
Respiratory Tract Infections
/
Sodium
/
Spleen
/
Diatrizoate Meglumine
/
Enzyme-Linked Immunosorbent Assay
/
Chlamydia
/
Blotting, Western
/
Chlamydophila pneumoniae
Type of study:
Diagnostic study
Limits:
Animals
/
Humans
Language:
Korean
Journal:
Korean Journal of Infectious Diseases
Year:
1997
Type:
Article
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