Tanshinone IIA, an ingredient of Salvia miltiorrhiza BUNGE, induces apoptosis in human leukemia cell lines through the activation of caspase-3
Experimental & Molecular Medicine
;
: 174-178, 1999.
Article
in English
| WPRIM
| ID: wpr-158709
ABSTRACT
Tanshinone II-A is a derivative of phenanthrene-quinone isolated from Salvia miltiorrhiza BUNGE, a traditional herbal medicine that is known to induce antiinflammatory, anti-oxidative and cytotoxic activity. We have examined cellular effects of Tanshione II-A on HL60 human promyelocytic leukemic cells and K562 human erythroleukemic cells. Tanshione II-A induced a dose- and time-dependent DNA fragmentation into the multiples of 180 bp and specific proteolytic cleavage of poly(ADP-ribose) polymerase in both cell lines. PI-staining and flow cytometry analysis of K562 cells following Tanshione II-A treatment showed an increase of the cells possessing hypodiploid DNA indicative of apoptotic state of cells. Caspase-3 activity was significantly increased during Tanshinone II-A treatment of both HL60 and K562 cells, whereas caspase-1 activity was not changed. These results suggest that Tanshione II-A induced HL60 and K562 cellular apoptosis that may be associated with the selective members of caspase family. Copyright 2000 Academic Press.
Full text:
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Index:
WPRIM (Western Pacific)
Main subject:
Phenanthrenes
/
Drugs, Chinese Herbal
/
Tumor Cells, Cultured
/
Leukemia
/
Leukemia, Erythroblastic, Acute
/
Cell Cycle
/
Apoptosis
/
HL-60 Cells
/
Lamiaceae
/
Caspases
Limits:
Humans
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
1999
Type:
Article
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