Induction of proliferation in resting B-cells by a factor released by activated mouse spleen cells
Experimental & Molecular Medicine
;
: 199-204, 1998.
Article
in English
| WPRIM
| ID: wpr-159769
ABSTRACT
Mouse spleen cells activated in a mixed lymphocyte reaction release a soluble factor, which induces a significant proliferative response in fresh mouse spleen cells. This proliferation inducing factor (PIF) was found to be heat stable (90 degrees C for 45 min) and also resistant to trypsin or chymotrypsin treatment. By using a sizing HPLC column, the molecular weight of PIF appears to be 25 kDa. Mouse spleen cells treated with anti-thy-1 + complement lost Con-A induced proliferative responses but responded well to PIF. B cell depleted spleen cells obtained by negative selection panning, did not respond to PIF. These results indicate that B cells proliferated in response to PIF. Polymixin-B, which blocks the B cell proliferative response to LPS, did not inhibit PIF induced proliferation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polymyxin B
/
Protein Denaturation
/
Spleen
/
Thymus Gland
/
Bone Marrow
/
B-Lymphocytes
/
Chymotrypsin
/
Trypsin
/
Cell Division
/
Chromatography, High Pressure Liquid
Limits:
Animals
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
1998
Type:
Article
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