Gene expression profiling of mouse aborted uterus induced by lipopolysac charide / 대한해부학회지
Anatomy & Cell Biology
;
: 98-105, 2011.
Article
in English
| WPRIM
| ID: wpr-159931
ABSTRACT
To identify genes that participate in the abortion process, normal pregnant uteri were compared to lipopolysaccharide (LPS)-induced abortion uteri. At day 6 of pregnancy, mice were treated with LPS at various time points to induce an abortion. Total RNAs were applied to a cDNA microarray to analyze genes with altered expression. At the early stage (2 hours) of LPS-induced abortion, upregulated genes were mainly composed of immune responsive genes, including Ccl4, Ccl2, Cxcl13, Gbp3, Gbp2, Mx2, H2-Eb1, Irf1 and Ifi203. Genes related to toll-like receptor signaling were also overexpressed. At late stages of abortion (12-24 hours), many genes were suppressed rather than activated, and these were mainly related to the extracellular matrix, cytoskeleton, and anti-apoptosis. Altered expression of several selected genes was confirmed by real time reverse transcription-polymerase chain reaction. The results demonstrated that many known genes were altered in the LPS-treated pregnant uterus, implying that the molecular mechanisms of the genes involved in LPS-induced abortion are complicated. Further analysis of this expression profile will help our understanding of the pathophysiological basis for abortion.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Uterus
/
Cytoskeleton
/
RNA
/
Gene Expression
/
Oligonucleotide Array Sequence Analysis
/
Gene Expression Profiling
/
Toll-Like Receptors
/
Extracellular Matrix
Limits:
Animals
/
Pregnancy
Language:
English
Journal:
Anatomy & Cell Biology
Year:
2011
Type:
Article
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