Inoculation route-dependent effects of BCG on asthmatic airway histology / 대한내과학회지
Korean Journal of Medicine
;
: 418-428, 2006.
Article
in Korean
| WPRIM
| ID: wpr-160202
ABSTRACT
BACKGROUND:
Intranasal (i.n.), as compared with subcutaneous (s.c.), BCG vaccination causes a greater suppression of airway eosinophilia. A comprehensive examination is needed to confirm that in various asthma models.METHODS:
BALB/c mice were immunized with i.n. or s.c. inoculation of BCG 1 x 10(5) CFUs. Sensitization and provocaton using ovalbumin (OVA) or Dermatophagoides farinae (Der f) were started at the same time or 1 week after the immunization. And then, a bronchoalveolar lavage (BAL) and an examination of lung tissue using a computerized image analyzer program were performed.RESULTS:
Both the i.n.- and s.c.- BCG infections reduced eosinophilia in both the BAL fluids and the lung tissues of both OVA- and Der f- asthma models. The proportions of BAL fluid lymphocyte in the mice infected with i.n. BCG were significantly lower than those with s.c. BCG (1.60+/-0.39% vs. 3.42+/-0.37%, p1,000 micrometer) airways 17.4+/-3.2 vs 37.0+/-5.9/mm2, p<0.05]. The goblet cell proportions in epithelium were also significantly lower in the mice received s.c.- as compared with i.n.- BCG (0.29+/-0.18 vs 0.43+/-0.20, p<0.01).CONCLUSIONS:
These results suggest that both i.n.- and s.c.- BCG inoculations reduce eosinophilia in airways, but the s.c. route is more effective in the suppression of the asthmatic responses in lung tissue.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Asthma
/
Lymphocytes
/
Ovalbumin
/
Immunization
/
Vaccination
/
Bronchoalveolar Lavage
/
Goblet Cells
/
Dermatophagoides farinae
/
Eosinophilia
/
Eosinophils
Limits:
Animals
Language:
Korean
Journal:
Korean Journal of Medicine
Year:
2006
Type:
Article
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