Adoptive Transfer of Colon Cancer Derived Peptide-specific CD8+ T Cells in HHD Mice
Immune Network
;
: 31-37, 2004.
Article
in Korean
| WPRIM
| ID: wpr-160486
ABSTRACT
BACKGROUND:
1-8D gene is a member of human 1-8 interferon inducible gene family and is shown to be overexpressed in fresh colon cancer tissues. Three peptides 1-6, 3-5 and 3-7 derived from 1-8D gene were shown to have immunogenicity against colon cancer.METHODS:
To study tumor immunotherapy of these peptides we established an adoptive transfer model. D(b-/-)Xbeta2 microglobulin (beta2m) null mice transgenic for a chimeric HLA-A2.1/D(b)-beta2m single chain (HHD mice) were immunized with irradiated peptide-loaded RMA-S/HHD/B7.1 transfectants. Spleens were removed after last immunization, and splenocytes were re-stimulated in vitro. Lymphocytes from vaccinated HHD mice were transferred together with IL-2 to the tumor bearing nude mice that were challenged S.C. with the HCT/HHD/B7 colon carcinoma cell line that was found to grow in these mice.RESULTS:
Peptide 3-5 was found to be highly effective in CTL activity. Adoptively transferred anti-peptide 3-5 cytolytic T lymphocytes caused significant retardation in tumor growth.CONCLUSION:
This study shows that peptide 3-5 can be the most effective candidate for the vaccine of adoptive immunotherapy against colon cancer.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptides
/
Spleen
/
Lymphocytes
/
T-Lymphocytes
/
Cell Line
/
Immunotherapy, Adoptive
/
Interferons
/
Immunization
/
Interleukin-2
/
Colon
Type of study:
Prognostic study
Limits:
Animals
/
Humans
Language:
Korean
Journal:
Immune Network
Year:
2004
Type:
Article
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