Immunization of mice with recombinant P27/30 protein confers protection against hard tick Haemaphysalis longicornis (Acari: Ixodidae) infestation
Journal of Veterinary Science
;
: 47-51, 2005.
Article
in English
| WPRIM
| ID: wpr-160619
ABSTRACT
The success of immunological control methods is dependent upon the use of potential key antigens as tick vaccine candidates. Previously, we cloned a gene encoding 27 kDa and 30 kDa proteins (P27/30) of Haemaphysalis longicornis, and identified the P27/30 is a troponin I-like protein. In this study, the recombinant P27/30 (rP27/30) expressed in Escherichia coli was used to immunize mice and the mice were challenge-infested with ticks at different developmental stages of the same species. The rP27/30 protein stimulated a specific protective anti-tick immune response in mice, evidenced by the statistically significant longer pre-feeding periods in adult ticks. Furthermore, significantly longer feeding periods were noted in both larval and adult ticks. On the other hand, only larval ticks exhibited low attachment rates (31.1%). Immunization of mice with rP27/30 protein confers protection against hard tick Haemaphysalis longicornis infestation. These results demonstrated that the rP27/30 protein might be a useful vaccine candidate antigen for biological control of ticks.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Tick Infestations
/
Recombinant Proteins
/
Vaccines, Synthetic
/
Ixodidae
/
Feeding Behavior
/
Mice, Inbred BALB C
/
Microfilament Proteins
Limits:
Animals
Language:
English
Journal:
Journal of Veterinary Science
Year:
2005
Type:
Article
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