Reduced Autophagy in 5-Fluorouracil Resistant Colon Cancer Cells
Biomolecules & Therapeutics
;
: 315-320, 2017.
Article
in English
| WPRIM
| ID: wpr-160698
ABSTRACT
We investigated the role of autophagy in SNUC5/5-FUR, 5-fluorouracil (5-FU) resistant SNUC5 colon cancer cells. SNUC5/5-FUR cells exhibited low level of autophagy, as determined by light microscopy, confocal microscopy, and flow cytometry following acridine orange staining, and the decreased level of GFP-LC3 puncta. In addition, expression of critical autophagic proteins such as Atg5, Beclin-1 and LC3-II and autophagic flux was diminished in SNUC5/5-FUR cells. Whereas production of reactive oxygen species (ROS) was significantly elevated in SNUC5/5-FUR cells, treatment with the ROS inhibitor N-acetyl cysteine further reduced the level of autophagy. Taken together, these results indicate that decreased autophagy is linked to 5-FU resistance in SNUC5 colon cancer cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Autophagy
/
Acridine Orange
/
Reactive Oxygen Species
/
Colon
/
Colonic Neoplasms
/
Microscopy, Confocal
/
Cysteine
/
Flow Cytometry
/
Fluorouracil
/
Microscopy
Language:
English
Journal:
Biomolecules & Therapeutics
Year:
2017
Type:
Article
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