A Study on the Calcium-Binding Protein in Gallbladder Bile / 대한내과학회지
Korean Journal of Medicine
;
: 470-475, 1997.
Article
in Korean
| WPRIM
| ID: wpr-160825
ABSTRACT
OBJECTIVES:
Calcium salts are usually present in the center of all types of gallstones, including pure cholesterol stones. It has been postulated that precipitation of calcium salts might act as a nidus for gallstone formation. Our goal was to determine whether a calcium-binding protein was present in bile and whether this protein has any calcium-binding properties.METHODS:
Calcium-binding moiety was obtained by addition of CaC12 into the gallbladder bile. Calcium-binding protein was identified by SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis). The capacity to bind calcium was confirmed by autoradiography with 45Ca++.RESULTS:
We identified a protein(M.W.CONCLUSION:
The presence of calcium-binding protein in bile and its ability to bind calcium suggest that it may play a functional role in the pathogenesis of gallstones.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Salts
/
Autoradiography
/
Bile
/
Gallstones
/
Calcium
/
Cholesterol
/
Electrophoresis, Polyacrylamide Gel
/
Gallbladder
Type of study:
Prognostic study
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Medicine
Year:
1997
Type:
Article
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