Neurotoxicity Screening in a Multipotent Neural Stem Cell Line Established from the Mouse Brain
Journal of Korean Medical Science
;
: 440-448, 2010.
Article
in English
| WPRIM
| ID: wpr-161033
ABSTRACT
Neural stem cells (NSCs) have mainly been applied to neurodegeneration in some medically intractable neurologic diseases. In this study, we established a novel NSC line and investigated the cytotoxic responses of NSCs to exogenous neurotoxicants, glutamates and reactive oxygen species (ROS). A multipotent NSC line, B2A1 cells, was established from long-term primary cultures of oligodendrocyte-enriched cells from an adult BALB/c mouse brain. B2A1 cells could be differentiated into neuronal, astrocytic and oligodendroglial lineages. The cells also expressed genotypic mRNA messages for both neural progenitor cells and differentiated neuronoglial cells. B2A1 cells treated with hydrogen peroxide and L-buthionine-(S,R)-sulfoximine underwent 30-40% cell death, while B2A1 cells treated with glutamate and kainate showed 25-35% cell death. Cytopathologic changes consisting of swollen cell bodies, loss of cytoplasmic processes, and nuclear chromatin disintegration, developed after exposure to both ROS and excitotoxic chemicals. These results suggest that B2A1 cells may be useful in the study of NSC biology and may constitute an effective neurotoxicity screening system for ROS and excitotoxic chemicals.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phenotype
/
Brain
/
Cell Differentiation
/
Cell Line
/
Neuroglia
/
Cytokines
/
Oxidants
/
Reactive Oxygen Species
/
Glutamic Acid
/
Excitatory Amino Acid Agonists
Type of study:
Diagnostic study
/
Screening study
Limits:
Animals
/
Humans
Language:
English
Journal:
Journal of Korean Medical Science
Year:
2010
Type:
Article
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