BCL-2 and BAX Expression in Normal Human Endometrium / 대한불임학회지

ABSTRACT

OBJECTIVE: To investigate the distribution of BCL-2, BAX proteins and DNA fragmented cells in the normal human endometrium during at each menstrual cycle in order to find out whether apoptosis regulates cyclic endometrial change. METHODS: Normal endometrial tissues wee obtained from 40 patients, 32~45 year of age, all with regular menstrual cycle, who were undergoing abdominal hysterectomy for myoma of uterus or cervical intraepithelial neoplasia for period from 1992 through 1997. Immunohistochemical staining was used to determine the expression of BCL-2 and BAX protein with paraffin-embedded tissues. RESULTS: BCL-2 was expressed on the glandular epithelial cells and stromal cells during the proliferative phase. The intensity of BCL-2 was increased predominantly on the basal layer than the functional layer in late proliferative phase. However, BCL-2 immunoreactivity was decreased in the secretory phase. BAX wes expressed predominantly during the secretory phase. the intensity was increased in late secretory phase rather than early secretory phase. DNA fragmented cells were detected in a few cells at each phase. However, it was increased during the late secretory phase. CONCLUSION: Apoptosis-related genes, BCL-2 and BAX, may play a role in the regulation of cyclic endometrial change.