The Regulation of MMP-2 and -14 Expressions by TGF-beta in Lens Epithelial Cells
Journal of the Korean Ophthalmological Society
;
: 1110-1116, 2006.
Article
in Korean
| WPRIM
| ID: wpr-161313
ABSTRACT
PURPOSE:
TGF-beta is a key regulator of epithelial-mesenchymal transition. Among the TGF-beta responses, cell migration is closely associated with the expression of matrix metalloproteinases (MMPs). Therefore, we determined which MMPs are regulated by TGF-beta and examined the TGF-beta signaling involved in this event, focusing on Src family tyrosine kinases (SFKs)METHODS:
First we examined the expression of MMPs in rat lens explant culture treated with TGF-beta and LECs attached to the anterior capsules of patients with nuclear (N), anterior polar (AP) cataracts using RT-PCR and immunofluorescence staining. It was examined whether the expression of MMPs is regulated by SFKs.RESULTS:
The study using RT-PCR and immunofluorescence staining showed the expression of MMP-2 and -14 in explants and the expression of MMP-14 LECs of AP cataracts. The expression of MMP-2 and -14 was blocked by PP2 in explants. Furthermore, the activated form of SFKs was observed in LECs of AP cataracts by immunofluorescence staining.CONCLUSIONS:
We suggest a novel role of SFKs signaling in the expression of MMP-14 induced by TGF-beta.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Cataract
/
Capsules
/
Cell Movement
/
Transforming Growth Factor beta
/
Fluorescent Antibody Technique
/
Src-Family Kinases
/
Matrix Metalloproteinases
/
Epithelial Cells
/
Epithelial-Mesenchymal Transition
Limits:
Animals
/
Humans
Language:
Korean
Journal:
Journal of the Korean Ophthalmological Society
Year:
2006
Type:
Article
Similar
MEDLINE
...
LILACS
LIS