Influence of Activin and Follistatin on Expression of Insulin-like Growth Factor (IGF)-I, II, Insulin-like Growth Factor Binding Protein (IGFBP)-1, 2, and 3 mRNA in Cultured Mouse Granulosa Cells / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology
;
: 592-599, 2003.
Article
in Korean
| WPRIM
| ID: wpr-161659
ABSTRACT
OBJECTIVE:
To investigate the influence of activin and follistatin on the expression of IGF (insulin-like growth factor)-I, II, IGFBP (insulin-like growth factor binding protein)-1, 2, and 3 mRNA in cultured mouse granulosa cells MATERIALS ANDMETHODS:
The granulosa cells were obtained from the mouse and cultured for 6 days with 10 ng/ml of activin, 10 ng/ml of follistatin, and 10 ng/ml of activin with 10 ng/m of follistatin, respectively. The cells not treated with activin or follistatin served as control. Reverse transcription-polymerase chain reaction (RT-PCR) has been used to examine the expression of IGF-I, II, IGFBP-1, 2, and 3 mRNA. Results were analyzed with Kolmogorov-Smirnov test and analysis of variance (ANOVA) and statistical significance was defined as p<0.05.RESULTS:
The expression of IGF-I and II mRNA were not different significantly. However, the expression of IGFBP-3 mRNA was significantly increased in the follistatin group compared to the control group (p<0.05) and significantly decreased in the activin with follistatin group compared to the control group (p<0.05). The expression of IGFBP-1 mRNA was seemed to be increased in the activin group and decreased in the follistatin group compared to the control group, respectively (p=0.07, p=0.07). The expression of IGFBP-2 and 3 mRNA were seemed to be decreased in the activin group compared to the control group (p=0.06, p=0.07, respectively).CONCLUSION:
Activin and follistatin might play a role as regulators of mouse ovarian physiology by modulating the IGF system, especially IGFBPs.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
RNA, Messenger
/
Insulin-Like Growth Factor I
/
Carrier Proteins
/
Insulin-Like Growth Factor Binding Proteins
/
Insulin-Like Growth Factor Binding Protein 1
/
Insulin-Like Growth Factor Binding Protein 2
/
Insulin-Like Growth Factor Binding Protein 3
/
Activins
/
Follistatin
Limits:
Animals
Language:
Korean
Journal:
Korean Journal of Obstetrics and Gynecology
Year:
2003
Type:
Article
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