Detection of 12 Respiratory Viruses with Two-set Multiplex Reverse Transcriptase-PCR Assay Using a Dual Priming Oligonucleotide System / 대한진단검사의학회지
The Korean Journal of Laboratory Medicine
;
: 420-427, 2007.
Article
in English
| WPRIM
| ID: wpr-161974
ABSTRACT
BACKGROUND:
We intended to evaluate the diagnostic usefulness of a multiplex reverse transcriptase- PCR (mRT-PCR) assay kit under dual priming oligonucleotide system (DPO) for the childhood acute respiratory tract infections.METHODS:
Two hundred nasopharyngeal aspirates were taken from children < or = 5 yr old admitted due to acute respiratory infections in 2004. Direct fluorescent antibody (FA) assays were performed with fresh specimens; then, mRT-PCRs for the detection of 12 respiratory viruses (Seeplex RV detection kit, SeeGene, Seoul, Korea) were tested with frozen specimens.RESULTS:
FA assays for five common respiratory viruses showed positive results in 66 patients (33.0%), while mRT-PCR detected causative viruses in 112 patients (56.0%), including 16 co-infected cases (8.0%). A total of 129 viruses were identified respiratory syncytial virus A/B (38.0%/7.8%), influenza virus A/B (10.1%/5.4%), parainfluenza virus 1/2/3 (7.0%/3.1%/7.8%), coronavirus 229E or NL63 (6.2%), human metapneumovirus (4.7%), adenovirus (4.7%), rhinovirus (3.9%), and coronavirus OC43 (1.6%).CONCLUSIONS:
DPO-based mRT-PCR was found as a sensitive tool for the detection of the viruses that cause childhood respiratory infections. Clinical significances of the agents detected by mRTPCR need further evaluations.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Respiratory Tract Infections
/
Viruses
/
Virus Diseases
/
DNA, Viral
/
Oligonucleotide Probes
/
Reproducibility of Results
/
Fluorescent Antibody Technique, Direct
/
Reverse Transcriptase Polymerase Chain Reaction
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Child, preschool
/
Humans
/
Infant
/
Infant, Newborn
Language:
English
Journal:
The Korean Journal of Laboratory Medicine
Year:
2007
Type:
Article
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