Distribution of T lymphocyte Subpopulations in Psoriatic Skin / 대한피부과학회지
Korean Journal of Dermatology
;
: 435-442, 1997.
Article
in Korean
| WPRIM
| ID: wpr-162245
ABSTRACT
BACKGROUND:
Although the main features of psoriasis consist of abnormal epidermal proliferation and T cell infiltration, which of these is the initial abnormality has been a longstanding unresolved question. Recently there has been definite evidence that activated T cells produce the cytokines that induce keratinocyte abnormalities.OBJECTIVE:
We investigated the distributions and relative numbers of T lymphocyte subpopulations, that is, CD4+ and CD8+ T cells, to verify the more important T cell subtype and its infiltrating site in the formation of psoriatic lesions.METHODS:
Paired psoriatic lesional and non-lesional skin tissues were obtained from eight typical psoriatic patients. Immunohistochemical staining was done on the frozen sections using a labelled streptavidin-biotin peroxidase complex method with DAKO LSAB kit. The primary antibodies used in this study were monoclonal or polyclonal antibodies against CD3, CD4, CD8, HLA-DR, and ICAM-1.RESULTS:
In lesional psoriatic skin, the epidermis was mainly infiltrated by CD8+ T cells. There were little or no T cells in non-lesional epidermis. In both lesional and non-lesional dermis, CD4+ T cells were the main ones. In lesional skin, anti-ICAM-1 antibody positively stained diffusely in the endothelial cells of papillary and subpapillary plexuses and focally in epidermal keratinocytes, but in non-lesional skin only the endothelial cells in the subpapillary plexus were stained. There were many HLA-DR+T lymphocytes in lesional and non-lesional dermis. In the epidermis, HLA DR was detected only in lesional keratinocytes and T lymphocytes.CONCLUSION:
These results suggest (1) participation of activated epidermal CD8+ T lymphocytes in the formation of psoriatic plaques, and (2) a pathogenetic role of ICAM-1 expression on papillary microvessels.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Psoriasis
/
Skin
/
Lymphocytes
/
T-Lymphocytes
/
HLA-DR Antigens
/
Keratinocytes
/
Cytokines
/
Lymphocyte Subsets
/
Peroxidase
/
Intercellular Adhesion Molecule-1
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Dermatology
Year:
1997
Type:
Article
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