TRPC3 cation channel plays an important role in proliferation and differentiation of skeletal muscle myoblasts
Experimental & Molecular Medicine
;
: 614-627, 2010.
Article
in English
| WPRIM
| ID: wpr-162255
ABSTRACT
During membrane depolarization associated with skeletal excitation-contraction (EC) coupling, dihydropyridine receptor [DHPR, a L-type Ca2+ channel in the transverse (t)-tubule membrane] undergoes conformational changes that are transmitted to ryanodine receptor 1 [RyR1, an internal Ca2+-release channel in the sarcoplasmic reticulum (SR) membrane] causing Ca2+ release from the SR. Canonical-type transient receptor potential cation channel 3 (TRPC3), an extracellular Ca2+-entry channel in the t-tubule and plasma membrane, is required for full-gain of skeletal EC coupling. To examine additional role(s) for TRPC3 in skeletal muscle other than mediation of EC coupling, in the present study, we created a stable myoblast line with reduced TRPC3 expression and without alpha1SDHPR (MDG/TRPC3 KD myoblast) by knock-down of TRPC3 in alpha1SDHPR-null muscular dysgenic (MDG) myoblasts using retrovirus-delivered small interference RNAs in order to eliminate any DHPR-associated EC coupling-related events. Unlike wild-type or alpha1SDHPR-null MDG myoblasts, MDG/TRPC3 KD myoblasts exhibited dramatic changes in cellular morphology (e.g., unusual expansion of both cell volume and the plasma membrane, and multi-nuclei) and failed to differentiate into myotubes possibly due to increased Ca2+ content in the SR. These results suggest that TRPC3 plays an important role in the maintenance of skeletal muscle myoblasts and myotubes.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Sarcoplasmic Reticulum
/
Calcium Channels
/
Cations
/
Cell Differentiation
/
Cells, Cultured
/
Calcium
/
Synaptophysin
/
Muscle Fibers, Skeletal
/
Ryanodine Receptor Calcium Release Channel
/
Calcium Channels, L-Type
Limits:
Animals
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
2010
Type:
Article
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