Synthetic CDCA Derivatives-Induced Apoptosis of Stomach Cancer Cell Line SNU-1 Cells / Journal of the Korean Cancer Association, 대한암학회지
Cancer Research and Treatment
;
: 132-139, 2004.
Article
in English
| WPRIM
| ID: wpr-162441
ABSTRACT
PURPOSE:
This study was conducted to explore whether CDCA derivatives induce apoptosis in a stomach cancer cell line, and to dissect the detailed mechanism underlying apoptosis. MATERIALS ANDMETHODS:
The human stomach cancer cell line, SNU-1, cells were treated with the synthetic CDCA derivatives, HS-1199 and HS-1200. DNA and mitochondrial stains were used to detect apoptotic cells by fluorescence imaging or flow cytometry. The caspase-3 activity was measured by Western blotting.RESULTS:
Both the HS-1199 and HS-1200 induced decreased viabilities of the SNU-1 cells, in time-dependent manners. The CDCA derivatives demonstrated various apoptosis hallmarks, such as mitochondrial changes reduction of MMP, cytochrome c release, and Smac/ DIABLO translocation), activation of caspase-3 (resulting in the degradation of PARP and DFF45), DNA fragmentation and nuclear condensation.CONCLUSION:
The CDCA derivatives, HS-1199 and HS- 1200, both induced apoptosis of the SNU-1 gastric cancer cells in caspase- and mitochondria-dependent fashions. Many important issues relating to their therapeutic applications remain to be elucidated.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Stomach
/
Stomach Neoplasms
/
DNA
/
Cell Line
/
Chenodeoxycholic Acid
/
Blotting, Western
/
Apoptosis
/
Caspases
/
Cytochromes c
/
Coloring Agents
Limits:
Humans
Language:
English
Journal:
Cancer Research and Treatment
Year:
2004
Type:
Article
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