Diagnostic Utility of Multiprobe Fluorescence in situ Hybridization Assay for Detecting Cytogenetic Aberrations in Acute Leukemia
Annals of Laboratory Medicine
;
: 198-202, 2014.
Article
in English
| WPRIM
| ID: wpr-163734
ABSTRACT
BACKGROUND:
Specific cytogenetic aberrations detected by conventional karyotyping or FISH play a major role in the diagnosis, prognosis, and treatment of patients with acute leukemia. The FISH technique enhances the capacity of conventional karyotyping to detect subtle chromosomal aberrations. Multiprobe FISH assay (Cytocell, UK) can hybridize multiple probes to a single slide, thereby increasing the detection rate of cytogenetic aberrations. This study aimed to evaluate multiprobe FISH in detecting cytogenetic abnormalities in acute leukemia.METHODS:
Thirty newly diagnosed acute leukemia patients who attended the hematology clinic at Dong-A University Hospital from October 2008 to October 2012 were enrolled in the study. The multiprobe FISH results were compared with those of G-banding.RESULTS:
Multiprobe FISH detected the chromosomal aberrations identified by G-banding, as well as additional aberrations in 6 of 30 (20.0%) cases, which included ETV6/RUNX1 translocation, p16 deletion, TP53 deletion, and IGH break-apart.CONCLUSIONS:
The multiprobe FISH assay was a more sensitive and reliable technique compared with G-banding. It was also more cost-effective and yielded faster results.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Repressor Proteins
/
Translocation, Genetic
/
Leukemia, Myeloid, Acute
/
Leukemia
/
Acute Disease
/
Tumor Suppressor Protein p53
/
Chromosome Banding
/
In Situ Hybridization, Fluorescence
/
Gene Deletion
/
Proto-Oncogene Proteins c-ets
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Adolescent
/
Adult
/
Aged
/
Child
/
Child, preschool
/
Humans
/
Male
Language:
English
Journal:
Annals of Laboratory Medicine
Year:
2014
Type:
Article
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