Proteomic Identification of Proteins Suggestive of Immune-Mediated Response or Neuronal Degeneration in Serum of Achalasia Patients
Gut and Liver
;
: 411-416, 2013.
Article
in English
| WPRIM
| ID: wpr-163966
ABSTRACT
BACKGROUND/AIMS:
The primary pathophysiologic abnormality in achalasia is known to be a loss of inhibitory myenteric ganglion cells, which may result from an immune-mediated response or neuronal degeneration. The aim of this study was to identify proteins suggestive of an immune-mediated response or neuronal degeneration in the serum of achalasia patients using a proteomic analysis.METHODS:
Blood samples were collected from five symptomatic achalasia patients and five sex- and age-matched healthy controls. Serum proteomic analysis was conducted, and the protein spots were identified using matrix-assisted laser desorption ionization/time-of-flight and a proteomics analyzer. The serum level of C3 was measured by enzyme-linked immunosorbent assay in nine patients with achalasia and 18 sex- and age-matched healthy controls.RESULTS:
Of the 658 matched protein spots, 28 spots were up-regulated over 2-fold in the serum from achalasia patients compared with that from controls. The up-regulated proteins included complement C4B5, complement C3, cyclin-dependent kinase 5, transthyretin, and alpha 2 macroglobulin. The serum levels of C3 in achalasia patients were significantly higher than those of controls.CONCLUSIONS:
The serum proteomic analysis of achalasia patients suggests an immune-mediated response or neuronal degeneration. Further validation studies in larger samples and the esophageal tissue of achalasia patients are required.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Alpha-Macroglobulins
/
Complement System Proteins
/
Complement C3
/
Prealbumin
/
Enzyme-Linked Immunosorbent Assay
/
Proteins
/
Esophageal Achalasia
/
Proteomics
/
Ganglion Cysts
/
Cyclin-Dependent Kinase 5
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
Gut and Liver
Year:
2013
Type:
Article
Similar
MEDLINE
...
LILACS
LIS