Comparison of an Automated Repetitive Sequence-based PCR Microbial Typing System with IS6110-Restriction Fragment Length Polymorphism for Epidemiologic Investigation of Clinical Mycobacterium tuberculosis Isolates in Korea / 대한진단검사의학회지
The Korean Journal of Laboratory Medicine
;
: 282-284, 2011.
Article
in English
| WPRIM
| ID: wpr-164048
ABSTRACT
Tuberculosis remains a severe public health problem worldwide. Presently, genotyping is used for conducting epidemiologic and clinical studies on tuberculosis cases. We evaluated the efficacy of the repetitive sequence-based PCR (rep-PCR)-based DiversiLab(TM) system (bioMerieux, France) over the IS6110-restriction fragment length polymorphism analysis for detecting Mycobacterium tuberculosis. In all, 89 clinical M. tuberculosis isolates collected nationwide from Korea were used. The DiversiLab system allocated the 89 isolates to 8 groups with 1 unique isolate when a similarity level of 95% was applied. Seventy-six isolates of the Beijing family and 13 isolates of non-Beijing family strains were irregularly distributed regardless of rep-PCR groups. The DiversiLab system generated a rapid, sensitive, and standardized result. It can be used to conduct molecular epidemiologic studies to identify clinical M. tuberculosis isolates in Korea.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Reagent Kits, Diagnostic
/
Automation
/
Tuberculosis
/
Polymorphism, Restriction Fragment Length
/
Repetitive Sequences, Nucleic Acid
/
Polymerase Chain Reaction
/
Epidemiologic Methods
/
Bacterial Typing Techniques
/
Republic of Korea
/
Genotype
Type of study:
Prognostic study
Limits:
Humans
Country/Region as subject:
Asia
Language:
English
Journal:
The Korean Journal of Laboratory Medicine
Year:
2011
Type:
Article
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