Isolation of GTP binding from bovine brain / 영남의대학술지
Yeungnam University Journal of Medicine
;
: 360-368, 1993.
Article
in Korean
| WPRIM
| ID: wpr-164068
ABSTRACT
GTP binding protein (G-protein) associated with membrane and involved in signal transduction was isolated from bovine brain, and molecular weight of G protein was observed. As the results, cell membranes were homogenized from bovine brain tissues and proteins of membrane were gained using 1% cholate, and progressed the chromatography. The purification process was performed by step, DEAE-Sephacel, Ulttrogel AcA 34 and heptylamine-Sepharose column chromatography. The chromatographic fractions were confirmed by GTP binding assay and SDS-polyacrylamide gel electrophoresis. Molecular weight of Goa was revealed 39,000 dalton and GR 36,000 dalton. One more step of heptylamine-Sepharose was enforced to purify the GTP binding protein. Finally I gained the GTP binding protein isolated subtype of Goalpha and Gbeta.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Brain
/
Signal Transduction
/
Cell Membrane
/
Chromatography
/
GTP-Binding Proteins
/
Cholates
/
Electrophoresis
/
Guanosine Triphosphate
/
Membranes
/
Molecular Weight
Language:
Korean
Journal:
Yeungnam University Journal of Medicine
Year:
1993
Type:
Article
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