Purification of the Protective Antigen from Bacillus anthracis
Journal of the Korean Society for Microbiology
;
: 589-594, 1998.
Article
in Korean
| WPRIM
| ID: wpr-164099
ABSTRACT
Anthrax toxin consists of three separate proteins, protective antigen (PA), edema factor (EF), and lethal factor (LF). PA binds to the receptor on mammalian cells and facilitates translocation of EF or LF into its cytosol. PA is the primary component of anthrax vaccines. In this study we purified PA from culture filtrates of Bacillus anthracis. The purification involved sequential chromatography through hydroxylapatite, DEAE-Sepharose CL-4B, followed by Mono-Q. The purified PA was judged to be homogeneous on SDS-PAGE, and consisted of a single polypeptide chain with a relative molecular weight of 85,000.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Bacillus
/
Bacillus anthracis
/
Chromatography
/
Durapatite
/
Anthrax Vaccines
/
Cytosol
/
Edema
/
Electrophoresis, Polyacrylamide Gel
/
Anthrax
/
Molecular Weight
Language:
Korean
Journal:
Journal of the Korean Society for Microbiology
Year:
1998
Type:
Article
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