The Production Mechanism of TNF-alpha and IL-6 by Group IIA Phospholipase A2 / 영남의대학술지
Yeungnam University Journal of Medicine
;
: 177-190, 2004.
Article
in Korean
| WPRIM
| ID: wpr-164554
ABSTRACT
BACKGROUND:
Secretory phospholipase A2 (sPLA2) are a group of extracellular enzymes that release fatty acids at the sn-2 position of phospholipids. Group IIA sPLA2 (sPLA2-IIA) has been detected in the inflammatory fluids, and its plasma level increases in the inflammatory disease. This study examined the effect of sPLA2-IIA on mouse macropahges in order to investigate the potential mechanism of sPLA2-induced inflammation. MATERIALS ANDMETHODS:
Wild type PLA2 and mutant H48Q PLA2 were purified from HEK293 cells transfected with the corresponding plasmids, and the PLA2 activities were measured using 1-palmitoyl-2-[1- (14) C]linoleoyl-3-phosphatidylethanolamine as substrates. The TNF-alpha and IL-6 released in the supernatants were determined by ELISA. In addition, the TNF-alpha and IL-6 mRNA were analyzed by RT-PCR.RESULTS:
sPLA2-IIA stimulated the production of TNF-alpha and IL-6 in a dose- and time-dependent manner. In addition, the effect of sPLA2-IIA on cytokine production from the macrophage was found to be associated with the accumulation of their specific mRNA. The mRNA levels of TNF-alpha and IL-6 peaked at 2 and 6 hours in a time-dependent manner, respectively.CONCLUSION:
In conclusion, the production of proinflammatory cytokine might be mediated by the binding of sPLA2-IIA to the receptors.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phospholipids
/
Plasma
/
Plasmids
/
RNA, Messenger
/
Enzyme-Linked Immunosorbent Assay
/
Interleukin-6
/
Tumor Necrosis Factor-alpha
/
Phospholipases A2, Secretory
/
Group II Phospholipases A2
/
Fatty Acids
Limits:
Animals
Language:
Korean
Journal:
Yeungnam University Journal of Medicine
Year:
2004
Type:
Article
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