Gingko biloba extract (EGb 761) attenuates ischemic brain injury-induced reduction in Ca2+ sensor protein hippocalcin / 한국실험동물학회지
Laboratory Animal Research
;
: 199-204, 2012.
Article
in English
| WPRIM
| ID: wpr-164973
ABSTRACT
Gingko biloba extract 761 (EGb 761) protects neuronal cells from ischemic brain injury via a number of neuroprotective mechanisms. Hippocalcin is a calcium sensor protein that regulates intracellular calcium concentrations and apoptotic cell death. We investigated whether EGb 761 regulates hippocalcin expression in cerebral ischemia. Male Sprague-Dawley rats were treated with vehicle or EGb 761 (100 mg/kg) prior to middle cerebral artery occlusion (MCAO), and cerebral cortex tissues were collected 24 h after MCAO. A proteomic approach demonstrated reduction in hippocalcin expression in vehicle-treated animals during MCAO, whereas EGb 761 treatment prevented injury-induced decreases in hippocalcin expression. RT-PCR and Western blot analyses indicated that EGb 761 attenuates injury-induced decrease in hippocalcin. These results suggest that the maintenance of hippocalcin during cerebral ischemia contributes to the neuroprotective role of EGb 761.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Brain
/
Brain Injuries
/
Plant Extracts
/
Cerebral Cortex
/
Brain Ischemia
/
Blotting, Western
/
Calcium
/
Cell Death
/
Rats, Sprague-Dawley
/
Ginkgo biloba
Limits:
Animals
/
Humans
/
Male
Language:
English
Journal:
Laboratory Animal Research
Year:
2012
Type:
Article
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