Your browser doesn't support javascript.
loading
Studying the Effect of Downregulating Autophagy-Related Gene LC3 on TLR3 Apoptotic Pathway Mediated by dsRNA in Hepatocellular Carcinoma Cells / Journal of the Korean Cancer Association, 대한암학회지
Cancer Research and Treatment ; : 230-245, 2017.
Article in English | WPRIM | ID: wpr-165948
ABSTRACT

PURPOSE:

The purpose of this study is to examine the role of the double-stranded RNA (dsRNA) activated Toll–interleukin-1 receptor domain-containing adaptor inducing interferon β (TRIF) signal pathway in triggering apoptosis in hepatocellular carcinoma (HCC) cells. MATERIALS AND

METHODS:

First, siRNA targeted autophagy–related gene LC3 (pU6H1-LC3 siRNA and siLC3) and a dsRNA used as a Toll-like receptor 3 (TLR3) ligand was constructed and synthesized, respectively. Then, a human HCC cell line was transfected with dsRNA, siLC3, and cotransfected with siLC3 and dsRNA (siLC3+dsRNA), respectively. Finally, quantification real-time polymerase chain reaction, western blotting, and immunofluorescence staining were used in the HCC line (SMMC7721), and MTT assay, flow cytometry, terminal deoxynucleotidyl transferase-mediated dUTP nick-end-labeling, and transmission electron microscopy were used in an HCC xenograft model of nude mice. Human umbilical vein endothelial cell tube forming assay, color Doppler ultrasonographic flow image examination, and CD34-positive microvessel density were used in vitro and in vivo.

RESULTS:

Compared with untreated cells, the protein and mRNA expression of TLR3 and TRIF was up-regulated, in order, siLC3+dsRNA, dsRNA, and siLC3. Expression of LC3 was obviously down-regulated and the autophagosomes were significantly decreased in siLC3+dsRNA and siLC3, whereas in dsRNA (p < 0.05). LC3 and TRIF colocation was observed in HepG2 cells. Decreased cell viability, increased apoptosis, decrease in xenograft tumor volume, and angiogenesis potential were also observed in order (p < 0.05).

CONCLUSION:

Suppression of intracellular autophagy resulted in decreased degradation of TRIF protein, which can promote triggering of apoptosis by the TLR3-TRIF pathway. dsRNA and siLC3 could play anticancer roles in coordination.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Autophagy / Umbilical Veins / In Vitro Techniques / RNA, Double-Stranded / RNA, Messenger / Signal Transduction / Cell Line / Cell Survival / Blotting, Western / Fluorescent Antibody Technique Limits: Animals / Humans Language: English Journal: Cancer Research and Treatment Year: 2017 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Autophagy / Umbilical Veins / In Vitro Techniques / RNA, Double-Stranded / RNA, Messenger / Signal Transduction / Cell Line / Cell Survival / Blotting, Western / Fluorescent Antibody Technique Limits: Animals / Humans Language: English Journal: Cancer Research and Treatment Year: 2017 Type: Article