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Fast temporal detection of intracellular hydrogen peroxide by HyPer
International Journal of Oral Biology ; : 169-173, 2013.
Article in Korean | WPRIM | ID: wpr-166282
ABSTRACT
HyPer is the genetically encoded biosensor of intracellular hydrogen peroxide (H2O2), the most stable of the reactive oxygen species (ROS) generated by living cells. HyPer has a high sensitivity and specificity for detecting intracellular H2O2 by confocal laser microscopy. However, it was not known whether high speed ratiometric imaging of H2O2 by HyPer is possible. We thus investigated the sensitivity of HyPer in detecting changes to the intracellular H2O2 levels in HEK293 and PC12 cells using a microfluorometer imaging system. Increase in the HyPer ratio were clearly evident on stimulations of more than 100 microM H2O2 and fast changes in the HyPer ratio were observed on ratiometric fluorescent images after H2O2 treatment. These results suggest that HyPer is a potent biosensor of the fast temporal production of intracellular H2O2.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Biosensing Techniques / Sensitivity and Specificity / PC12 Cells / Reactive Oxygen Species / Microscopy, Confocal / Hydrogen / Hydrogen Peroxide Type of study: Diagnostic study Limits: Animals Language: Korean Journal: International Journal of Oral Biology Year: 2013 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Biosensing Techniques / Sensitivity and Specificity / PC12 Cells / Reactive Oxygen Species / Microscopy, Confocal / Hydrogen / Hydrogen Peroxide Type of study: Diagnostic study Limits: Animals Language: Korean Journal: International Journal of Oral Biology Year: 2013 Type: Article