Direct Presumptive Identification of Candida species from Blood Cultures Using CHROMagar Candida / 대한임상병리학회지
Korean Journal of Clinical Pathology
;
: 128-136, 1997.
Article
in Korean
| WPRIM
| ID: wpr-166782
ABSTRACT
BACKGROUND:
CHROMagar Candida is a new differential medium that allows selective isolation and identification of clinically significant yeasts. We evaluated the use of this medium to identify Candida species directly from positive blood culture bottles.METHODS:
A total of 152 positive blood culture bottles (51 Candida albicans, 29 Candida troficalis, 28 Candida parapsilosis, 26 Candida glabrata, 10 Candida krusei, 4 Candida pelliculosa. 1 Candida guilliermonidii, 3 C. albicans plus C. glabrata) were directly subcultures to CHROMagar (Hardy diagnostics. USA) and incubated for 48 h. Colony appearance on CHROMagar was assessed independently by three observers.RESULTS:
CHROMagar correctly identified 95.4%, 92 1% and 91.4% of Candida app. from blood cultures by the three observers. respectively. There was 91.4% agree cent between the observers. Expected colony appearance on CHROMagar was 100% for C. albicans. 97.7% for C. tropicalis, 96.7% for C. krusei, 94 9% for C. glabrata but 88.1% for C. parapsilosis. Three mixed candidemias, not detected by conventional methods, were detected by CHROMagar.CONCLUSIONS:
CHROMagar permits earlier recognition of major Cardida app. in positive blood cultures and more reliable detection of mixed candidemias.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Yeasts
/
Candida
/
Candida albicans
/
Candida glabrata
/
Candidemia
Type of study:
Diagnostic study
Language:
Korean
Journal:
Korean Journal of Clinical Pathology
Year:
1997
Type:
Article
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