Proliferation Patterns of N-butyl-N- ( 4-hydroxybutyl ) Nitrosamine ( BBN ) Induced Rat Bladder Tumor Identified by Histone mRNA In Situ Hybridization / 대한비뇨기과학회지
Korean Journal of Urology
;
: 111-119, 1997.
Article
in Korean
| WPRIM
| ID: wpr-166830
ABSTRACT
The synthesis of histone mRNA is closely coupled with DNA replication. During the S-phase of the cell cycle, the level of histone mRNA increases over fifty fold then rapidly disappears at the start of G2-phase. The presence, therefore, of abundant quantities of histone mRNA provides a molecular marker of cycling cells. The expression of histone mRNA was investigated by in situ hybridization method in N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) induced rat bladder tumor to determine the proliferation patterns. This method resulted in intense brown/black cytoplasmic staining of cells containing histone mRNA and extent and intensity of stain were increased in tumorigenesis (normal, simple hyperplasia, nodular or papillary hyperplasia, and transitional cell carcinoma-Ta,T1). The higher S-phase fraction indicated greater biological malignancy based on the fact that the extent and intensity of stain progressively increased with tumorigenesis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Urinary Bladder
/
Urinary Bladder Neoplasms
/
RNA, Messenger
/
Histones
/
Cell Cycle
/
In Situ Hybridization
/
Cytoplasm
/
DNA Replication
/
Carcinogenesis
/
Hyperplasia
Limits:
Animals
Language:
Korean
Journal:
Korean Journal of Urology
Year:
1997
Type:
Article
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