The Expression of DNA Dependent Protein Kinase Protein in Malignant Lymphoma
Korean Journal of Pathology
;
: 477-485, 2001.
Article
in Korean
| WPRIM
| ID: wpr-166947
ABSTRACT
BACKGROUND:
DNA dependent protein kinase (DNA-PK) composed of Ku70, Ku80 and DNA-PK catalytic subunit (DNA-PKcs), plays an important role in the primary repair of break points of damaged DNA and is involved in the recombination of the V, D, and J genes. Loss of DNA-PK may lead to immunodeficiency and malignant lymphomas. This hypothesis has been supported by animal study in a Ku70 knock-out mouse model. The relationship between DNA-PK and human malignant lymphoma has not yet been studied.METHODS:
We examined the loss of DNA-PK proteins in 85 representative cases of different subtypes of malignant lymphoma. Immunohistochemical stains for DNA-PK subunits were performed on formalin-fixed and paraffin-embedded tissue sections.RESULTS:
Lymphomas demonstrated statistically significant loss of Ku70, Ku80 and DNA-PKcs. T cell lymphomas showed more loss of DNA-PK proteins in comparison with B cell lymphomas. According to the World Health Organization (WHO) classification system, both T cell lymphomas and high-grade subtypes of B cell lymphomas demonstrated similar degree of loss of DNA-PK proteins.CONCLUSIONS:
We confirmed the loss of DNA-PK proteins in malignant lymphomas through the results of our study, and the loss of these proteins seems to be more significant in high-grade lymphomas. These findings support the role of DNA-PK as a tumor suppressor gene.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Protein Kinases
/
Recombination, Genetic
/
World Health Organization
/
Lymphoma, Non-Hodgkin
/
DNA
/
Genes, Tumor Suppressor
/
Lymphoma, B-Cell
/
Lymphoma, T-Cell
/
Classification
/
Catalytic Domain
Type of study:
Prognostic study
Limits:
Animals
/
Humans
Language:
Korean
Journal:
Korean Journal of Pathology
Year:
2001
Type:
Article
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