Your browser doesn't support javascript.
loading
Protective mechanism of glucose against alloxan-induced beta-cell damage: pivotal role of ATP
Experimental & Molecular Medicine ; : 12-17, 2000.
Article in English | WPRIM | ID: wpr-16701
ABSTRACT
Glucose prevents the development of diabetes induced by alloxan. In the present study, the protective mechanism of glucose against alloxan-induced beta-cell damage was investigated using HIT-T 15 cell, a Syrian hamster transformed beta-cell line. Alloxan caused beta-cell damages with DNA fragmentation, inhibition of glucose-stimulated insulin release, and decrease of cellular ATP level, but all of these beta-cell damages by alloxan were prevented by the presence of 20 mM glucose. Oligomycin, a specific inhibitor of ATP synthase, completely abolished the protective effects of glucose against alloxan-induced cell damage. Furthermore, treatment of nuclei isolated from HIT-T15 cells with ATP significantly prevented the DNA fragmentation induced by Ca2+. The results indicate that ATP produced during glucose metabolism plays a pivotal role in the protection of glucose against alloxan-induced beta-cell damage.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Oligomycins / DNA / B-Lymphocytes / Cell Line / Cell Nucleus / Cell Survival / Adenosine Triphosphate / Calcium / Egtazic Acid / Dose-Response Relationship, Drug Language: English Journal: Experimental & Molecular Medicine Year: 2000 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Oligomycins / DNA / B-Lymphocytes / Cell Line / Cell Nucleus / Cell Survival / Adenosine Triphosphate / Calcium / Egtazic Acid / Dose-Response Relationship, Drug Language: English Journal: Experimental & Molecular Medicine Year: 2000 Type: Article