Protective mechanism of glucose against alloxan-induced beta-cell damage: pivotal role of ATP
Experimental & Molecular Medicine
;
: 12-17, 2000.
Article
in English
| WPRIM
| ID: wpr-16701
ABSTRACT
Glucose prevents the development of diabetes induced by alloxan. In the present study, the protective mechanism of glucose against alloxan-induced beta-cell damage was investigated using HIT-T 15 cell, a Syrian hamster transformed beta-cell line. Alloxan caused beta-cell damages with DNA fragmentation, inhibition of glucose-stimulated insulin release, and decrease of cellular ATP level, but all of these beta-cell damages by alloxan were prevented by the presence of 20 mM glucose. Oligomycin, a specific inhibitor of ATP synthase, completely abolished the protective effects of glucose against alloxan-induced cell damage. Furthermore, treatment of nuclei isolated from HIT-T15 cells with ATP significantly prevented the DNA fragmentation induced by Ca2+. The results indicate that ATP produced during glucose metabolism plays a pivotal role in the protection of glucose against alloxan-induced beta-cell damage.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oligomycins
/
DNA
/
B-Lymphocytes
/
Cell Line
/
Cell Nucleus
/
Cell Survival
/
Adenosine Triphosphate
/
Calcium
/
Egtazic Acid
/
Dose-Response Relationship, Drug
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
2000
Type:
Article
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