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Ectopic expression of cyclooxygenase-2-induced dedifferentiation in articular chondrocytes
Experimental & Molecular Medicine ; : 721-727, 2008.
Article in English | WPRIM | ID: wpr-167141
ABSTRACT
Cyclooxygenase-2 (COX-2) is known to modulate bone metabolism, including bone formation and resorption. Because cartilage serves as a template for endochondral bone formation and because cartilage development is initiated by the differentiation of mesenchymal cells into chondrocytes (Ahrens et al., 1977; Sandell and Adler, 1999; Solursh, 1989), it is of interest to know whether COX-2 expression affect chondrocyte differentiation. Therefore, we investigated the effects of COX-2 protein on differentiation in rabbit articular chondrocyte and chick limb bud mesenchymal cells. Overexpression of COX-2 protein was induced by the COX-2 cDNA transfection. Ectopic expression of COX-2 was sufficient to causes dedifferentiation in articular chondrocytes as determined by the expression of type II collagen via Alcian blue staining and Western blot. Also, COX-2 overexpression caused suppression of SOX-9 expression, a major transcription factor that regulates type II collagen expression, as indicated by the Western blot and RT-PCR. We further examined ectopic expression of COX-2 in chondrifying mesenchymal cells. As expected, COX-2 cDNA transfection blocked cartilage nodule formation as determined by Alcian blue staining. Our results collectively suggest that COX-2 overexpression causes dedifferentiation in articular chondrocytes and inhibits chondrogenic differentiation of mesenchymal cells.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Cartilage, Articular / Cell Differentiation / Cells, Cultured / Chondrocytes / Chondrogenesis / Collagen Type II / Cyclooxygenase 2 / Interleukin-1beta / SOX9 Transcription Factor / Mesenchymal Stem Cells Limits: Animals Language: English Journal: Experimental & Molecular Medicine Year: 2008 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Cartilage, Articular / Cell Differentiation / Cells, Cultured / Chondrocytes / Chondrogenesis / Collagen Type II / Cyclooxygenase 2 / Interleukin-1beta / SOX9 Transcription Factor / Mesenchymal Stem Cells Limits: Animals Language: English Journal: Experimental & Molecular Medicine Year: 2008 Type: Article