Molecular and phylogenetic analysis of Anaplasma spp. in sheep and goats from six provinces of China
Journal of Veterinary Science
;
: 523-529, 2016.
Article
in English
| WPRIM
| ID: wpr-167766
ABSTRACT
Members of the genus Anaplasma are important emerging tick-borne pathogens in both humans and animals in tropical and subtropical areas. Here, we investigated the presence of Anaplasma spp. in 621 sheep and 710 goats from six provinces of China. Polymerase chain reaction (PCR) and DNA sequencing were conducted to determine the prevalence of Anaplasma (A.) phagocytophilum, A. ovis and A. bovis targeting the 16S ribosomal RNA or the major surface protein 4 gene. PCR revealed Anaplasma in 39.0% (240/621) of sheep and 45.5% (323/710) of goats. The most frequently detected species was A. ovis (88/621, 14.2% for sheep; 129/710, 18.2% for goats), followed by A. bovis (60/621, 9.7% for sheep; 74/710, 10.4% for goats) and A. phagocytophilum (33/621, 5.3% for sheep; 15/710, 2.1% for goats). Additionally, eight sheep and 20 goats were found to be infected with three pathogens simultaneously. DNA sequencing confirmed the presence of these three Anaplasma species in the investigated areas, and phylogenetic analysis indicated that there was geographic segregation to a certain extent, as well as a relationship between the host and cluster of A. ovis. The results of the present study provide valuable data that helps understand the epidemiology of anaplasmosis in ruminants from China.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Ruminants
/
Goats
/
Sheep
/
RNA, Ribosomal, 16S
/
China
/
Polymerase Chain Reaction
/
Epidemiology
/
Prevalence
/
Sequence Analysis, DNA
/
Anaplasma phagocytophilum
Type of study:
Prevalence study
Limits:
Animals
/
Humans
Country/Region as subject:
Asia
Language:
English
Journal:
Journal of Veterinary Science
Year:
2016
Type:
Article
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