Effect of Cryotherapy on Focal Adhesion Kinase in Retinal Pigment Epithelial Cells
Journal of the Korean Ophthalmological Society
;
: 360-365, 2005.
Article
in Korean
| WPRIM
| ID: wpr-168177
ABSTRACT
PURPOSE:
In the pathogenesis of proliferative vitreoretinopathy (PVR), retinal pigment epithelial (RPE) cells do not undergo apoptosis after separation from the basement membrane. In order to investigate the mechanism by which RPE cells resist apoptosis in PVR, the expression of focal adhesion kinase after cryotherapy was studied.METHODS:
After lensectomy and vitrectomy was performed on rabbit eyes, a retinal tear was created. Cryotherapy was applied to the tear site one week after the tear creation. Rabbits were sacrificed at 1, 4 and 7 days after the tear formation and 30 minutes, 1, 4, 7, 9, 21 days after cryotherapy. Eyes were stained with anti-focal adhesion kinase (FAK) antibody.RESULTS:
Expression of FAK increased after the tear formation and remained increased until 21days after cryotherapy.CONCLUSIONS:
These data show that creation of a retinal tear and cryotherapy increases FAK expression and may contribute to resistance to apoptosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phosphotransferases
/
Retinal Perforations
/
Retinaldehyde
/
Basement Membrane
/
Vitrectomy
/
Apoptosis
/
Cryotherapy
/
Vitreoretinopathy, Proliferative
/
Focal Adhesions
/
Epithelial Cells
Limits:
Animals
Language:
Korean
Journal:
Journal of the Korean Ophthalmological Society
Year:
2005
Type:
Article
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