The Effect of Cigarette Smoking on the Expression of MMP-9, TIMP-1 and VEGF in Nasal Polyp Epithelial Cells and Fibroblasts
Journal of Rhinology
;
: 121-127, 2009.
Article
in Korean
| WPRIM
| ID: wpr-168421
ABSTRACT
BACKGROUND AND OBJECTIVES:
It has been known that MMP-9, TIMP-1, and VEGF play an important role in the formation of nasal polyps (NPs). The aim of this study is to investigate the effect of smoking on the expression of MMP-9, TIMP-1 and VEGF in the epithelial cells and fibroblasts of NPs. MATERIALS ANDMETHODS:
The epithelial cells and fibroblasts of NPs obtained from 10 patients who underwent endoscopic sinus surgery were cultured and used. The prepared CSE concentrations were 5%, 1%, 0.5% and 0.1%. In the control group, cells were cultured for 9 hours in the media containing 1 ml of AEGM and DMEM/F-12K. In the CSE group, cells were treated with 5%, 1%, 0.5% and 0.1% CSE for 9 hours. After washing with PBS, cells were cultured in medium for 24 hours. ELISA was performed to measure the secretion of MMP-9, TIMP-1 and VEGF proteins and real-time PCR was performed to assay their mRNAs.RESULTS:
The production of MMP-9, TIMP-1 and VEGF proteins did not change significantly in comparison with the control group in epithelial cells and fibroblasts after exposure to CSE.CONCLUSION:
These results suggest that CSE does not appear to have an effect on the expression of MMP-9, TIMP-1 and VEGF for developing and growth of nasal polyposis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Smoke
/
Enzyme-Linked Immunosorbent Assay
/
Proteins
/
Smoking
/
Nasal Polyps
/
Tissue Inhibitor of Metalloproteinase-1
/
Vascular Endothelial Growth Factor A
/
Epithelial Cells
/
Real-Time Polymerase Chain Reaction
/
Fibroblasts
Limits:
Humans
Language:
Korean
Journal:
Journal of Rhinology
Year:
2009
Type:
Article
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