The Effect of Lens Nucleus and Cortex Material on Lens Epithelial Cell Culture through In vitro Capsular Bag Model
Journal of the Korean Ophthalmological Society
;
: 630-637, 2001.
Article
in Korean
| WPRIM
| ID: wpr-168601
ABSTRACT
PURPOSE:
This study attempts to evaluate the effect of lens cortex and nucleus remnants on posterior capsular opacification with method of the cell culture according to in vitro capsular bag model.METHODS:
After bovine lens were isolated, we performed continuous curvilinear capsulorhexis and hydrodissectioin of the lens fiber mass. At this stage a tension ring was implanted and then the preparations placed in organ culture for up to 6 weeks. Lens cortex and nucleus material was added at the culture media in group 2, 3, 4, 5, 6 with amount of 1/16, 1/32, 1/64, 1/96, 1/128 of one lens volume. Group 1 was control group that was not added lens materials. Cell coverage of the posterior lens capsule was recorded and the capsules were examined, both pre-and post-coverage, for proliferative activity.RESULTS:
After a lag period outgrowth was observed across the posterior capsule. The proliferative activity was greater at the groups that were added more amount of the lens cortex and nucleus material.CONCLUSIONS:
it is important that we should not remain any lens cortex material remnant at cataract surgery.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Organ Culture Techniques
/
Cataract
/
Capsules
/
Cell Culture Techniques
/
Capsulorhexis
/
Culture Media
/
Epithelial Cells
Language:
Korean
Journal:
Journal of the Korean Ophthalmological Society
Year:
2001
Type:
Article
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