Polymerase Chain Reaction in the Diagnosis of Infectious Endophthalmitis
Journal of the Korean Ophthalmological Society
; : 600-606, 2001.
Article
in Ko
| WPRIM
| ID: wpr-168606
Responsible library:
WPRO
ABSTRACT
PURPOSE: We evaluated the efficacy of the PCR which is known as more sensitive method than culture in the diagnosis of causal microorganisms of the infective endophthalmitis. METHODS: We used 0.3 ml of aqueous humor and 0.5 ml of vitreous sample in 3 cases of postoperative and 1 case of endogenous endophthalmitis for detecting causal microorganisms. To detect the bacteria we used universal, Gram positive and negative primers, and to detect the fungus we used fungal primer. RESULTS: Three cases of endophthalmitis, there was no bacteria in the bacterial culture for 10 days but PCR results identified causal microorganisms in all cases. CONCLUSIONS: PCR is effective in the fast and accurate diagnosis of infective endophthalmitis and especially in the culture-negative endophthalmitis.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Aqueous Humor
/
Bacteria
/
Endophthalmitis
/
Polymerase Chain Reaction
/
Diagnosis
/
Fungi
Type of study:
Diagnostic_studies
Language:
Ko
Journal:
Journal of the Korean Ophthalmological Society
Year:
2001
Type:
Article