Sox9 Increases the Proliferation and Colony-forming Activity of Outer Root Sheath Cells Cultured In Vitro
Annals of Dermatology
;
: 138-143, 2011.
Article
in English
| WPRIM
| ID: wpr-168742
ABSTRACT
BACKGROUND:
beta-catenin plays a pivotal role in hair follicle development and hair growth cycle.OBJECTIVE:
The aim of this study was to identify beta-catenin-regulated genes in cultured human hair outer root sheath (ORS) cells.METHODS:
Primary cultured ORS cells were transduced with recombinant adenovirus expressing N-terminal truncated beta-catenin (constitutive active form), and beta-catenin-regulated genes were identified.RESULTS:
Overexpression of the constitutively active form of beta-catenin led to induction of Sox9 expression at both mRNA and protein levels. To investigate the potential role of Sox9, we made the recombinant adenovirus expressing green fluorescent protein-tagged Sox9, and then transduced into cultured ORS cells. Interestingly, Sox9 induced the expression of keratin 15, increased the proliferation of ORS cells in vitro, and enhanced colony-forming activity.CONCLUSION:
Our results suggest that Sox9 is a beta-catenin-regulated gene in ORS cells, and has potential importance in the regulation of hair follicle homeostasis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
RNA, Messenger
/
Adenoviridae
/
Hair Follicle
/
Electrolytes
/
Beta Catenin
/
Keratin-15
/
Hair
/
Homeostasis
Limits:
Humans
Language:
English
Journal:
Annals of Dermatology
Year:
2011
Type:
Article
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