Direct Measurement of High-Density Lipoprotein Cholesterol Evaluated / 대한임상병리학회지

ABSTRACT

BACKGROUND: Serum high density lipoprotein (HDL)-cholesterol level is used as an assessment of the risk of coronary heart disease. In this study, we evaluated direct measurement of HDL- cholesterol in serum with polyethylene-modified enzymes and sulfated alpha-cyclodextrin. METHODS: We evaluated the precision, the lower limit of detection, the recovery rate, the linearity, the interference for hemoglobin and the comparision with the result of HDL-cholesterol measured by selective precipitation method. We also studied the specificity of this direct method for very low density lipoprotein (VLDL) and low density lipoprotein (LDL). RESULTS: The total imprecision was 3.8% (low), 3.5% (middle), 3.2% (high). The lower limit of detection was 0 mg/L. The recovery rate was satisfactory. The linearity was also (r2=0.99). This method showed a good correlation (r2=0.97) with the selective precipitation method in HDL- cholesterol measurement. VLDL-cholesterol (up to 300 mg/L) increased HDL-cholesterol only less than 3% but increased VLDL-cholesterol to 400 mg/L, more than 750 mg/L caused 5% and 15% of overestimation of HDL-cholesterol, respectively. LDL-cholesterol (142-1,073 mg/L) increased or decreased HDL-cholesterol by some degree (about 15%). Hemoglobin (up to 3,000 mg/L) did not influence this assay. CONCLUSIONS: The direct measurement of HDL-cholesterol is satisfactory method in HDL- cholesterol measurement in good analytical performance and may be anticipated to reduce workload of laboratory because the sample pretreatment is not necessary.