Molecular Epidemiological Characteristics of Drug-resistant Mycobacterium tuberculosis by Random Amplified Polymorphic DNA Genotyping / 대한임상병리학회지
Korean Journal of Clinical Pathology
;
: 274-281, 2001.
Article
in Korean
| WPRIM
| ID: wpr-168881
ABSTRACT
BACKGROUND:
In spite of appropriate therapy and control for tuberculosis, the prevalence of tuberculosis is still frequent in Korea. Emerging infection and rapid detection of multidrug-resistant Mycobacterium tuberculosis (MDR-TB) are major interests in microbiologic laboratories. In this study, we evaluated the usefulness of random amplified polymorphic DNA (RAPD) genotyping for molecular epidemiological characteristics of MDR-TB.METHODS:
We analyzed 64 clinical strains of M. tuberculosis including 35 strains which showed resistance to one or more antimycobacterial drugs and M. tuberculosis H37Rv (ATCC 27294), as a drug-sensitive control strain. RAPD genotyping analysis was carried out under eight reaction conditions and using ten random primers (A-1245, AP-50, B-1245, DKU-44, DKU-49, Leg-1, INS-2, IS-986-FP, PF-15 and MBR).RESULTS:
RAPD patterns using six primers (IS-986-FP, DKU-44, DKU-49, INS-2, B-1245, and AP-50) showed marked polymorphisms that were easier to discriminate than those with other primers. RAPD patterns represented various polymorphisms among 64 strains. However, RAPD could not discriminate MDR-TB strains from drug-sensitive ones.CONCLUSIONS:
RAPD genotyping is assumed a preferable technique for discrimination among clinical strains of M. tuberculosis but not for specifying MDR-TB strains.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Tuberculosis
/
DNA
/
Prevalence
/
Discrimination, Psychological
/
Korea
/
Mycobacterium
/
Mycobacterium tuberculosis
Type of study:
Controlled clinical trial
/
Prevalence study
Country/Region as subject:
Asia
Language:
Korean
Journal:
Korean Journal of Clinical Pathology
Year:
2001
Type:
Article
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