Utility of RARa Fluorescence In Situ Hybridization for Follow-up in Acute Promyelocytic Leukemia: Comparison with PML/RARa Fluorescence In Situ Hybridization / 대한임상병리학회지
Korean Journal of Clinical Pathology
;
: 253-259, 2001.
Article
in Korean
| WPRIM
| ID: wpr-168885
ABSTRACT
BACKGROUND:
The most noted rearrangement identified in acute promyelocytic leukemia (APL) involves the PML and RARa genes, which results in the formation of the PML/RARa gene fusion. In the fluorescence in situ hybridization (FISH) for PML/RARa, the two signals may coincidentally overlap in normal nuclei. We investigated whether a new RARa rearrangement probe could discriminate the false-positive fusion signal of the PML/RARa translocation probe.METHODS:
A total of 51 cases, which showed the results from 1% to the borderline level by PML/ RARa FISH, were re-tested with the RARa rearrangement probe. Also, we compared the RARa FISH with the PML/RARa FISH on 16 patients with newly diagnosed APL and performed the RARa FISH on 20 bone marrow specimens without hematologic malignancies in order to set up the cut-off value.RESULTS:
The cut-off for the RARa FISH was determined as 1.02%. For patients with newly diagnosed APL, the PML/RARa FISH showed positive signals in 95.3+/-6.5% of the cells and RARa FISH showed positive signals in 97.0+/-7.0% (r=0.83). Of a total of 41 cases consisting of hematological disorders other than APL, five cases showed results equal to or greater than 5% with PML/RARa FISH and one case showed a positive result with RARa FISH. Of 10 follow-up APL cases, seven cases showed results equal to or greater than 5% with the PML/RARa FISH and four cases showed positive results with the RARa FISH.CONCLUSIONS:
The cut-off value for the RARa FISH is 1.02% and we consider RARa FISH as the proper method for follow-up of APL.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Bone Marrow
/
Leukemia, Promyelocytic, Acute
/
Follow-Up Studies
/
In Situ Hybridization
/
Hematologic Neoplasms
/
Gene Fusion
/
Fluorescence
Type of study:
Observational study
/
Prognostic study
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Clinical Pathology
Year:
2001
Type:
Article
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