Inductoin of Radioresistance by Overexpression of Glutathione S-Transferase K1 (hGSTK1) in MCF-7 Cells / 대한방사선종양학회지
The Journal of the Korean Society for Therapeutic Radiology and Oncology
;
: 381-388, 2001.
Article
in Korean
| WPRIM
| ID: wpr-16895
ABSTRACT
PURPOSE:
This study was conducted to assess the effects of x-irradiation on the expression of the novel glutathione S-transferase K1 gene. MATERIALS ANDMETHODS:
Human glutathione S-transferase K1 (hGSTK1) DNA was purified and ligated to a pcDNA3.1/Myc-His(+) vector for the overexpression of hGSTK1 gene. MCF-7 cells were transfected with or without the recombinant hGSTK1 gene, and irradiated with 6 MV x-ray. After incubation of 14 days, cell survival was measured and compared. The expression of hGSTK1 and the effect of x- irradiation on hGSTK1 expression were also estimated in MCF-7 cells transfected with or without the hGSTK1 gene by RT-PCR.RESULTS:
Following 2 to 12 Gy of x-irradiation, the cell survivals were higher in the MCF-7 cells transfected with the hGSTK1 gene than in those without transfection. Despite the higher cell survival in the hGSTK1-transfected cells, RT-PCR for hGSTK1 mRNA revealed no significant differences according to radiation dose, fractionation, and time after irradiation.CONCLUSION:
The MCF-7 cells transfected with the hGSTK1 gene showed higher cell survival than those without transfection of the gene. The hGSTK1 gene might be associated with the radiosensitivity of MCF-7 cell line and further analysis should be needed.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Radiation Tolerance
/
DNA
/
RNA, Messenger
/
Transfection
/
Cell Survival
/
MCF-7 Cells
/
Glutathione
/
Glutathione Transferase
Limits:
Humans
Language:
Korean
Journal:
The Journal of the Korean Society for Therapeutic Radiology and Oncology
Year:
2001
Type:
Article
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