Quercetin induces cell death by caspase-dependent and p38 MAPK pathway in EGFR mutant lung cancer cells / 고신대학교의과대학학술지
Kosin Medical Journal
;
: 30-40, 2016.
Article
in English
| WPRIM
| ID: wpr-169014
ABSTRACT
OBJECTIVES:
The aim of this study was whether quercetin induces cell death by caspase and MAPK signaling pathway in EGFR mutant lung cancer cells.METHODS:
PC-9 cells, EGFR mutant lung cancer cells, were treated various times and concentrations of quercetin and harvested and measured using MTT assay, DNA fragmentation, Western blotting, and FACS analysis.RESULTS:
Treatment with quercetin in PC-9 cells resulted in inhibition of cell growth through apoptosis. Quercetin-induced apoptosis was associated with caspase-dependent manner. Quercetin also significantly increased levels of phosphor-p38 and decreased levels of phosphor-ERK, indicating that quercetin induces p38 MAPK signaling pathway in PC-9 cells. Quecetin treatment also generated the release of cytochrome c in PC-9 cells; however, pretreatment with rotenone or z-LEHD-fmk, significantly attenuated quercetin-induced apoptosis.CONCLUSIONS:
Our data indicate that quercetin exhibits EGFR mutant lung cancer effects through apoptosis by caspase dependent and mitochondrial pathway.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Quercetin
/
Rotenone
/
Blotting, Western
/
Cell Death
/
Apoptosis
/
Cytochromes c
/
P38 Mitogen-Activated Protein Kinases
/
DNA Fragmentation
/
Lung
/
Lung Neoplasms
Language:
English
Journal:
Kosin Medical Journal
Year:
2016
Type:
Article
Similar
MEDLINE
...
LILACS
LIS