Double-stranded RNA virus in Korean Isolate IH-2 of Trichomonas vaginalis
The Korean Journal of Parasitology
; : 87-94, 2007.
Article
in En
| WPRIM
| ID: wpr-169040
Responsible library:
WPRO
ABSTRACT
In this study, we describe Korean isolates of Trichomonas vaginalis infected with double-stranded (ds) RNA virus (TVV). One T. vaginalis isolate infected with TVV IH-2 evidenced weak pathogenicity in the mouse assay coupled with the persistent presence of a dsRNA, thereby indicating a hypovirulence effect of dsRNA in T. vaginalis. Cloning and sequence analysis results revealed that the genomic dsRNA of TVV IH-2 was 4,647 bp in length and evidenced a sequence identity of 80% with the previously-described TVV 1-1 and 1-5, but only a 42% identity with TVV 2-1 and 3 isolates. It harbored 2 overlapping open reading frames of the putative capsid protein and dsRNA-dependent RNA polymerase (RdRp). As previously observed in the TVV isolates 1-1 and 1-5, a conserved ribosomal slippage heptamer (CCUUUUU) and its surrounding sequence context within the consensus 14-nt overlap implied the gene expression of a capsid protein-RdRp fusion protein, occurring as the result of a potential ribosomal frameshift event. The phylogenetic analysis of RdRp showed that the Korean TVV IH-2 isolate formed a compact group with TVV 1-1 and 1-5 isolates, which was divergent from TVV 2-1, 3 and other viral isolates classified as members of the Giardiavirus genus.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Phylogeny
/
Trichomonas Infections
/
Trichomonas vaginalis
/
Virulence
/
RNA-Dependent RNA Polymerase
/
RNA, Double-Stranded
/
RNA, Viral
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Molecular Sequence Data
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Open Reading Frames
/
Cloning, Molecular
Limits:
Animals
/
Female
/
Humans
Country/Region as subject:
Asia
Language:
En
Journal:
The Korean Journal of Parasitology
Year:
2007
Type:
Article