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Genetic diversity of porcine reproductive and respiratory syndrome virus in Korea
Journal of Veterinary Science ; : 115-124, 2013.
Article in English | WPRIM | ID: wpr-169637
ABSTRACT
The high genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) has been an obstacle to developing an effective vaccine for porcine reproductive and respiratory syndrome (PRRS). This study was performed to assess the degree of genetic diversity among PRRSVs from Korean pig farms where wasting and respiratory syndrome was observed from 2005 to 2009. Samples from 786 farms were tested for the presence of PRRSV using reverse transcription PCR protocol. A total of 117 farms were positive for type 1 PRRSV while 198 farms were positive for type 2. Nucleotide sequences encoding the open reading frame (ORF) 5 were analyzed and compared to those of various published PRRSV isolates obtained worldwide. Sequence identity of the ORF 5 in the isolates was 81.6~100% for type 1 viruses and 81.4~100% for type 2 viruses. Phylogenetic analysis of the ORF 5 sequences showed that types 1 and 2 PRRSVs from Korea were mainly classified into three and four clusters, respectively. The analyzed isolates were distributed throughout the clusters independent of the isolation year or geographical origin. In conclusion, our results indicated that the genetic diversity of PRRSVs from Korean pig farms is high and has been increasing over time.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Phylogeny / Swine / Genetic Variation / Open Reading Frames / Sequence Analysis, DNA / Porcine respiratory and reproductive syndrome virus / Porcine Reproductive and Respiratory Syndrome / Reverse Transcriptase Polymerase Chain Reaction / Sequence Analysis, Protein / Republic of Korea Limits: Animals Country/Region as subject: Asia Language: English Journal: Journal of Veterinary Science Year: 2013 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Phylogeny / Swine / Genetic Variation / Open Reading Frames / Sequence Analysis, DNA / Porcine respiratory and reproductive syndrome virus / Porcine Reproductive and Respiratory Syndrome / Reverse Transcriptase Polymerase Chain Reaction / Sequence Analysis, Protein / Republic of Korea Limits: Animals Country/Region as subject: Asia Language: English Journal: Journal of Veterinary Science Year: 2013 Type: Article