A Comparison of Expressions of MMP-3, MMP-7, TIMP-1 and Apoptosis in Keratoacanthoma and Squamous Cell Carcinoma / 대한피부과학회지
Korean Journal of Dermatology
;
: 489-495, 2005.
Article
in Korean
| WPRIM
| ID: wpr-169831
ABSTRACT
BACKGROUND:
Keratoacanthoma (KA) is a unique neoplasm, usually accompanied by rapid growth and regression, and the histologic findings resemble those of squamous cell carcinoma (SCC). Histologic differentiation of KA and SCC is challenging, therefore a number of studies have been carried out to differentiate these two entities using immunohistochemical staining. However, the results were inconsistent. There is now a debate as to whether KA is a benign tumor or a less aggressive variant of SCC.OBJECTIVE:
The purpose of this study was to examine the expression patterns of MMP-3, MMP-7, TIMP-1, the apoptotic index in KA and SCC, and the usefulness of immunohistochemical staining in differentiating these two entities.METHODS:
Formalin-fixed, paraffin-embedded biopsy specimens obtained from 12 KA and 16 SCC patients were investigated for the expressions of MMP-3, MMP-7 and TIMP-1 using an immnohistochemical staining method and apoptotic index by TUNEL method.RESULTS:
1. The expression of MMP-3 in mass was slightly higher in the SCC group (3.4+/-1.03) than the KA group (2.7+/-1.50). The expression of MMP-3 on the stroma was significantly higher for the SCC group (2.6+/-0.96) than the KA group (1.5+/-1.24) (p<0.05). 2. The expression of MMP-7 was detected only in a single case within the SCC group. 3. The expression of TIMP-1 was detected in two cases within the KA group. 4. The apoptotic index (%) was significantly higher in the SCC group (57.3+/- 32.15) than the KA group (36.1+/-21.78) (p<0.05).CONCLUSION:
The immunohistochemical staining method of MMP-3, MMP-7 and TIMP-1 is not sensitive enough to differentiate between the two diseases. However, it has been suggested that MMP-3 of the stroma is involved in invasion of SCC more than MMP-3 of the mass, that MMP-7 plays a role in the early stage of SCC, and that TIMP-1 inhibits the action of MMPs and invasion in KA. We suggest that KA can be regarded as a clinically benign end of the SCC disease spectrum. The apoptotic index was higher in the SCC group than the KA group, implying that SCC has greater invasive ability due to a newly-selected, mutated, neoplastic cellular clone that has more aggressive biological behavior.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Biopsy
/
Carcinoma, Squamous Cell
/
Clone Cells
/
Apoptosis
/
Tissue Inhibitor of Metalloproteinase-1
/
In Situ Nick-End Labeling
/
Matrix Metalloproteinases
/
Keratoacanthoma
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Dermatology
Year:
2005
Type:
Article
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