Skin Organ Culture Model for Evaluation of Melanin Pigmentation / 대한피부과학회지
Korean Journal of Dermatology
;
: 450-454, 2005.
Article
in Korean
| WPRIM
| ID: wpr-169837
ABSTRACT
BACKGROUND:
Skin organ culture is widely used as a tool to investigate skin biology or skin disease.OBJECTIVE:
The objective of the present study was to develop an ideal skin organ culture model for evaluation of melanin pigmentation.METHODS:
An air-liquid interface and submerged method were used. The histology of the cultured skin was studied with H&E stain. To examine the epidermal pigmentation, Fontana-Masson stain and NKI/beteb stain were performed. Pigment modifiers (arbutin, LY294002) were applied to the culture medium for 3 days as an air-liquid interface culture.RESULTS:
The general architecture of the skin was well maintained for 5 days. The melanin pigment decreased during culture without change of the number of melanocytes. As expected from previous reports, the effect of pigment modifiers (arbutin, LY294002) on cultured skin was demonstrated.CONCLUSION:
The results indicate that this skin organ culture model is useful in evaluating the melanin pigmentation
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Organ Culture Techniques
/
Skin
/
Skin Diseases
/
Biology
/
Pigmentation
/
Melanins
/
Melanocytes
Language:
Korean
Journal:
Korean Journal of Dermatology
Year:
2005
Type:
Article
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