The Study on Vitrification and Ultrarapid Thawing of Human Embryonic Stem Cells / 대한불임학회지
Korean Journal of Fertility and Sterility
;
: 13-20, 2002.
Article
in Korean
| WPRIM
| ID: wpr-170176
ABSTRACT
OBJECTIVE:
This study was carried out to establish the effectiveness of the vitrification method and the optimal cryoprotectants in the cryopreservation of human embryonic stem cells (ESC). MATERIALS ANDMETHODS:
Human ESC clumps established at Seoul National University Hospital (SNUhES 1) were cryopreserved with the vitrification method using the EM grid. EDS and EFS40 were used as vitrification solutions.RESULTS:
Between the EDS and EFS40 groups, there was no significant difference in the recovery rate after cryopreservation of human ESC. The formation rates of ESC colonies in the vitrified groups were significantly lower than those in the control ESC group (p<0.05, p<0.05). In addition, the formation rate of ESC colonies in the EDS group was significantly higher than that in the EFS40 group (p<0.05). The ESC colonies in the vitrified groups were significantly smaller after culture duration of 2 and 4 days, respectively, compared with the control ESC group (p<0.01, p<0.05). However, these effects could be reduced to nonsignificant level by the additional culture of ESC colonies. The vitrified human ESC retained the properties of pluripotent cells, including the expression of cell surface markers for the undifferentiated cells such as alkaline phosphatase and SSEA-4 (stage-specific embryonic antigen-4), and the expression of transcription factor Oct-4 (octamer-binding transcription factor-4), and the normal karyotype.CONCLUSION:
The vitrification method using the EM grid and EDS solution was confirmed to be very effective for the cryopreservation of human ESC.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Cryopreservation
/
Alkaline Phosphatase
/
Octamer Transcription Factor-3
/
Embryonic Stem Cells
/
Vitrification
/
Karyotype
/
Seoul
Limits:
Humans
Country/Region as subject:
Asia
Language:
Korean
Journal:
Korean Journal of Fertility and Sterility
Year:
2002
Type:
Article
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